By exposing cells and tissues to UV light, the excitation of naturally occurring chromophores occurs in part by non-radiative deactivations, in part via fluorescence emission.
Using prostatic abscess complications induced autofluorescence LIAF in natural tissues not impregnated with photosensitizers as a noninvasive autofluorescence technique for both diagnosis and intraoperative assessments of laryngeal cancer we can improve the tumor malign identification in vivo.
A total of three laryngeal biopsies i.
The samples were collected from patients previously diagnosed with stage T3 laryngeal carcinoma. Immediately after the surgery fragments of normal tissue and neoplastic tissue were collected, fragments of which later, after freezing, were sectioned in μm thickness slices and stretched to quartz slides.
The signals were recorded using a spectrograph and a photo-sensor module, the output of which was fed to a digital oscilloscope.
We assessed the impact of laser induced autofluorescence and autofluorescence lifetime measurements in order to identify the differences between healthy and tumoral laryngeal tissue and outlining them, in terms of differences between the laser autoinduced fluorescence averaged intensity.
The results determined the usefulness of laser induced spectroscopy in the diagnosis of laryngeal squamous cell carcinoma, discriminating between the malignant and normal tissue by analyzing the differences in spectral autofluorescence intensity and autofluorescence prostatic abscess complications.
The authors thank Prof. Pascu for permanent support in performing this research. Number CXIX - 1,